Image from Google Jackets

Nucleic Acid Detection of Plant Genes Using CRISPR-Cas13.

Tipo de material: TextoTextoSeries ; The CRISPR Journal,, 2(3), p.165-171, 2019Trabajos contenidos:
  • Abudayyeh, O.O
  • Gootenberg, J.S
  • Kellner, M.J
  • Zhang,F
Tema(s): Recursos en línea: Resumen: Nucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identi?cation. Here, we use a modi?ed version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in a mixture of soybeans and to detect multiple plant genes in a single reaction. SHERLOCK is rapid (*15min), quantitative, and portable, and can process crude soybean extracts as input material for minimal nucleic acid sample preparation. This ?eld-ready SHERLOCK platform with color-based lateral ?ow readout can be applied for detection and quantitation of genes in a range of agricultural applications.
Tags from this library: No tags from this library for this title. Log in to add tags.
Star ratings
    Average rating: 0.0 (0 votes)
Holdings
Item type Current library Collection Call number Status Date due Barcode
Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-17819 (Browse shelf(Opens below)) Available

Nucleic acid detection is vital for agricultural applications including trait detection during breeding, pest surveillance, and pathogen identi?cation. Here, we use a modi?ed version of the CRISPR-based nucleic acid detection platform SHERLOCK to quantify levels of a glyphosate resistance gene in a mixture of soybeans and to detect multiple plant genes in a single reaction. SHERLOCK is rapid (*15min), quantitative, and portable, and can process crude soybean extracts as input material for minimal nucleic acid sample preparation. This ?eld-ready SHERLOCK platform with color-based lateral ?ow readout can be applied for detection and quantitation of genes in a range of agricultural applications.

There are no comments on this title.

to post a comment.