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Molecular-sieve electrophoresis in cross-linked polyacrylamide gels

Tipo de material: TextoTextoSeries ; Journal of Chromatography A, 11, p.66-70, 1963Trabajos contenidos:
  • Hjertén, S
Tema(s): Recursos en línea: Resumen: The migration velocity of a protein in cross-linked polyacrylamide gels is dependent not only on the charge which the protein carries, but also to a large extent on the molecular size of the protein. The latter factor is so pronounced that reversal of the migration velocities of two proteins of different molecular weights can often be accomplished by mere changes in the gel concentration, i.e. the pore size. If this "molecular-sieve" electrophoresis is combined with electrophoresis in a medium without molecular sieving properties a high resolution can be expected. "Molecular-sieve" electrophoresis, which can be used both for analytical and small scale preparative purposes, can in some cases give separations similar to those obtained in "molecular-sieve" chromatography.
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The migration velocity of a protein in cross-linked polyacrylamide gels is dependent not only on the charge which the protein carries, but also to a large extent on the molecular size of the protein. The latter factor is so pronounced that reversal of the migration velocities of two proteins of different molecular weights can often be accomplished by mere changes in the gel concentration, i.e. the pore size. If this "molecular-sieve" electrophoresis is combined with electrophoresis in a medium without molecular sieving properties a high resolution can be expected. "Molecular-sieve" electrophoresis, which can be used both for analytical and small scale preparative purposes, can in some cases give separations similar to those obtained in "molecular-sieve" chromatography.

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