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Construction and analysis of infectious transcripts synthesized from full-length cDNA clones of both genomic RNAs of pea early browning virus.

Tipo de material: TextoTextoSeries ; Virology, 182(1), p.124-129, 1991Trabajos contenidos:
  • Macfarlane, S. A
  • Wallis, C. V
  • Taylor, S. C
  • Goulden, M. G
  • Wood, K. R
  • Davies, J. W
Tema(s): Recursos en línea: Resumen: Full-length cDNA clones of both RNAs of pea early browning virus have been constructed. Synthetic transcripts derived in vitro from these clones are infectious when inoculated onto plants. Electron microscopy revealed the presence of virions in transcript-inoculated plants, and both purified RNA and virions isolated from such plants could be used to infect other plants. Transcripts of RNA1 alone were able to replicate and spread systemically which is a characteristic of members of the tobravirus group of plant viruses.
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Documentos solicitados Documentos solicitados CICY Documento préstamo interbibliotecario Ref1 B-18189 (Browse shelf(Opens below)) Available

Full-length cDNA clones of both RNAs of pea early browning virus have been constructed. Synthetic transcripts derived in vitro from these clones are infectious when inoculated onto plants. Electron microscopy revealed the presence of virions in transcript-inoculated plants, and both purified RNA and virions isolated from such plants could be used to infect other plants. Transcripts of RNA1 alone were able to replicate and spread systemically which is a characteristic of members of the tobravirus group of plant viruses.

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