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Effect of ethylenediaminetetraacetic acid (EDTA)on growth from spores of Bacillus cereus.

Tipo de material: TextoTextoSeries ; Journal of Food Science, 50(3), p.661-664., 1985Trabajos contenidos:
  • Bulgarelli, M. A
  • Shelef, L. A
Tema(s): Recursos en línea: Resumen: Germination and growth from spores of toxigenic B. cereus strain USDA 201 in BHI broth containing Na2EDTA were studied. Reduced growth was effected by low EDTA concentrations (< 300 ppm). Little or no growth was observed after incubation for 48 hr in broth containing from 300-1,000 ppm of EDTA EDTA did not affect percent spore germination, or release of Ca following heat activation and subsequent incubation. However, delayed colony development was observed as EDTA concentrations increased in the broth, and atypically small colonies formed on Plate Count Agar. Growth inhibition by 500 ppm EDTA was evident over the pH range 5-9, with the highest spore resistance observed at pH 7. Addition of Fe, Zn, and Ca to the media reversed the growth inhibitory action, whereas Mg was less effective
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Germination and growth from spores of toxigenic B. cereus strain USDA 201 in BHI broth containing Na2EDTA were studied. Reduced growth was effected by low EDTA concentrations (< 300 ppm). Little or no growth was observed after incubation for 48 hr in broth containing from 300-1,000 ppm of EDTA EDTA did not affect percent spore germination, or release of Ca following heat activation and subsequent incubation. However, delayed colony development was observed as EDTA concentrations increased in the broth, and atypically small colonies formed on Plate Count Agar. Growth inhibition by 500 ppm EDTA was evident over the pH range 5-9, with the highest spore resistance observed at pH 7. Addition of Fe, Zn, and Ca to the media reversed the growth inhibitory action, whereas Mg was less effective

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