In vitro regeneration of Phaseolus vulgaris L. via direct and indirect organogenesis

A stable and efcient regeneration system is a necessary condition for the genetic transformation of Phaseolus vulgarisL. In this study, cotyledonary nodes with one cotyledon, cotyledonary nodes with two cotyledons, embryonic axes and root segments of 5-day-old germinated seeds were used as explants to evaluate explants to induce shoots and calli. Besides, using cotyledonary nodes with one cotyledon as explants, the efects of diferent concentrations of auxins and cytokinins on the direct and indirect organogenesis of common bean were investigated. As a result, embryonic axes and cotyledonary nodes with two cotyledons were the most efective explant types for the formation of shoots and calli. The cotyledonary nodes with one cotyledon were cultured in the shoot induction medium containing 7 mg L-16-benzyladenine (BAP)and 0.2 mg L -1a-naphthaleneacetic acid (NAA). 74.21