Antioxidant and antifungal profiles of phenol acid rich-fractions from Sida urens l. Against mycelia growth inhibition of some aspergillus and fusarium species

Objective: To verify the antifungal capacity of Sida urens L.(Malvaceae)traditionally used against mycelia growth inhibition of some Aspergillus and Fusarium species affecting maize and certain crops or the man by mycotoxins such as aflatoxins responsible of cancers. Methods: The antioxidant activity of phenol acid-rich fractions has been evaluated using ?-carotene-linoleic acid system, 1,1-diphenyl-2-picrylhydrazyl inhibitory activity and chelation of iron (II)ions which showed more pronounced antioxidant capacities of Sida urens L. The antifungal effect of phenol acid-rich fractions was studied using Minimum inhibitory concentration (MIC), Minimum fungicidal concentration (MFC)determined by the microdilution and at last the evaluation of Fractional Inhibitory Concentration Index (FICI). Results: Phenol acid-rich fractions from Sida urens L. exhibit maximum radical scavenging activity. As for the Minimum inhibitory concentration assay (MIC)and Minimum fungicidal concentration (MFC)of extract and their combination with standard fungicide (Nystatin), result varied according to microorganism. About FICI, the results indicate a synergistic effect between extract and the standard fungicide (Nystatin). Conclusions: The results indicated that the phenol acid-rich fraction possessed antioxidant and antifungal properties, and may give credence to some of its ethnopharmacological uses. Therefore, phenol acid-rich fraction of Sida urens L., can be effectively employed as antimicrobial agents, specifically antifungal to control growth and prevent colonization and spoilage of food and other plant products with the attendant financial losses by fungi, and prevent the cancers due to the mycotoxins such as aflatoxins.