The complete genome sequence of cucumopine-type Agrobacterium rhizogenes strain K599 (NCPPB2659), a nature's genetic engineer inducing hairy roots

Tipo de material: Texto

TextoSeries ; International Journal of Agriculture & Biology, 20(5), p.1167-1174, 2018Trabajos contenidos:

Tong, X
Li, Y
Xiang, T
Chen, Z
Wang, L
Zhang, C
Wu, L

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Resumen: Agrobacterium rhizogenes induces hairy roots from the infection site, when transferred DNA (T-DNA)in Ri plasmid inserted and integrated into host plant cell genome. Cucumopine-type A. rhizogenes strain K599 (NCPPB2659)has particularly high ability to induce hairy root. Here, using next-generation and third generation sequencing technologies, we do novo assembled the whole genome of K599. The genome is 5,114,385 bp and consists of one circular chromosome of 2,622,758 bp, one linear chromosome of 2,289,334 bp, and one Ri plasmid (pRi2659)of 202,292 bp. It contains 4716 protein-coding and 70 RNA genes, including 54 tRNAs, 12 rRNAs, and 4 ncRNAs. The genetic information of K599 exhibits the prediction of prophage regions and virulent genes, which is critical for DNA transfer to the host plant genome.

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Agrobacterium rhizogenes induces hairy roots from the infection site, when transferred DNA (T-DNA)in Ri plasmid inserted and integrated into host plant cell genome. Cucumopine-type A. rhizogenes strain K599 (NCPPB2659)has particularly high ability to induce hairy root. Here, using next-generation and third generation sequencing technologies, we do novo assembled the whole genome of K599. The genome is 5,114,385 bp and consists of one circular chromosome of 2,622,758 bp, one linear chromosome of 2,289,334 bp, and one Ri plasmid (pRi2659)of 202,292 bp. It contains 4716 protein-coding and 70 RNA genes, including 54 tRNAs, 12 rRNAs, and 4 ncRNAs. The genetic information of K599 exhibits the prediction of prophage regions and virulent genes, which is critical for DNA transfer to the host plant genome.

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