On the reptation theory of gel electrophoresis

Tipo de material: Texto

TextoSeries Biopolymers, 25(3), p.431-454, 1986Trabajos contenidos:

Slater, G. W
Noolandi, J

Tema(s):

GEL ELECTROPHORESIS

Recursos en línea:

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Resumen: We introduce a new biased reptation theory that allows a qualitative and semiquantitative study of DNA gel electrophoresis. We prove that stretching of the end-to-end vector of a very long charged reptating chain in an electric field occurs in a short period of time during a typical electrophoresis experiment, and that this leads to a fielddependent mobility only weaky dependent upon the size of the chain, in agreement with experiments on DNA. We also propose a practical technique to avoid chain stretching. Finally, many scaling laws are predicted, most of them being verifiable by experiment.

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Artículo

We introduce a new biased reptation theory that allows a qualitative and semiquantitative study of DNA gel electrophoresis. We prove that stretching of the end-to-end vector of a very long charged reptating chain in an electric field occurs in a short period of time during a typical electrophoresis experiment, and that this leads to a fielddependent mobility only weaky dependent upon the size of the chain, in agreement with experiments on DNA. We also propose a practical technique to avoid chain stretching. Finally, many scaling laws are predicted, most of them being verifiable by experiment.

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