Electrophoresis: Basic principle, types, and applications. In Basic Biotechniques for Bioprocess and Bioentrepreneurship

Tipo de material: Texto

TextoSeries Basic Biotechniques for Bioprocess and Bioentrepreneurship, p.183-193, 2023Trabajos contenidos:

Rana, B
Joshi, G.K

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Resumen: Electrophoresis refers to the separation of charged molecules based on their mobility in an electric field. This is a routinely used technique employed for various preparative and analytical purposes including separation, purification, and characterization of nucleic acids (DNA and RNA) and proteins. It is a simple, fast, and highly sensitive technique in which a mixture of charged molecules are allowed to migrate under the influence of an electric field towards the respective electrodes either freely or through a supportive medium such as gel with sieving property. While migrating, the separation of charged molecules from one another takes place depending upon the differences in their size, shape, and charge.

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Capítulo de Libro

Electrophoresis refers to the separation of charged molecules based on their mobility in an electric field. This is a routinely used technique employed for various preparative and analytical purposes including separation, purification, and characterization of nucleic acids (DNA and RNA) and proteins. It is a simple, fast, and highly sensitive technique in which a mixture of charged molecules are allowed to migrate under the influence of an electric field towards the respective electrodes either freely or through a supportive medium such as gel with sieving property. While migrating, the separation of charged molecules from one another takes place depending upon the differences in their size, shape, and charge.

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