Subcellular Proteomics [electronic resource] : From Cell Deconstruction to System Reconstruction / edited by Eric Bertrand, Michel Faupel.

Membrane Proteomics -- Keynotes on Membrane Proteomics -- Two-Dimensional BAC/SDS-PAGE for Membrane Proteomics -- Microparticles: A New Tool for Plasma Membrane Sub-cellular Proteomic -- Lipid Raft Proteomics: More than Just Detergent-Resistant Membranes -- Organelle Subproteomes -- Organelle Proteome Variation Among Different Cell Types: Lessons from Nuclear Membrane Proteins -- Synaptosome Proteomics -- Proteomic Analysis of Secreted Exosomes -- Characterization of Supramolecular Protein Complexes -- From Protein-Protein Complexes to Interactomics -- Supramolecular Signalling Complexes in the Nervous System -- Protein Networks and Complexes in Photoreceptor Cilia -- Subcellular Systems Biology -- Systems Biology and the Reconstruction of the Cell: From Molecular Components to Integral Function -- Automated, Systematic Determination of Protein Subcellular Location using Fluorescence Microscopy -- Systems Biology of the Endoplasmic Reticulum Stress Response -- Emerging Technologies in Proteomics -- Systems Nanobiology: From Quantitative Single Molecule Biophysics to Microfluidic-Based Single Cell Analysis -- Biophotonics Applied to Proteomics -- Differential Epitope Identification of Antibodies Against Intracellular Domains of Alzheimer's Amyloid Precursor Protein Using High Resolution Affinity-mass Spectrometry -- LC-MALDI MS and MS/MS - An Efficient Tool in Proteome Analysis.

This volume summarizes the new developments that made subcellular proteomics a rapidly expanding area. Recent success stories demonstrated that the combination of subcellular prefractionation methods with proteomic analysis is a very potent approach to simplify complex protein extracts from cells or tissues and to detect low abundance proteins. It also made clear that sophisticated strategies encompassing sample preparation, analytics and validation steps were required to fully exploit the potential of subcellular proteomics. Three sections are devoted to the different levels of subcellular organization and their specific methodologies. This is completed by a section on systems biology that deals with the integration of the data derived from these different levels to produce a synthetic description of the cell as a system. A survey of the most advanced technical developments in proteomics is also included, which covers single cell analysis methods, molecular imaging and LC-MALDI. Written by a panel of leading experts in the field, this book represents an unprecedented attempt to reflect state-of-the-art methodology and also to convey both the mode of thinking and the hype of this discipline. This volume should appeal not only to experts in the field and to researchers in disciplines such as cell biology or biochemistry but also to newcomers who wish to learn about the proteomic analysis of subcellular structures. Lastly, it can also serve as a reference guide for teachers, advanced undergraduates and graduate students desiring to learn more about this challenging field.