Molecular biomethods handbook

Incluye referencias bibliográficas e índice

Contenido: The manipulation of nucleic acids : basic tools and techniques / Gayle Corkill and Ralph Rapley -- Restriction enzymes : tools in clinical research / Gareth J.S. Jenkins -- Principles and medical applications of the polymerase chain reaction / Bimal D.M. Theophilus -- Probe design, production, and applications / Marilena Aquino de Muro -- Southern blotting as a diagnostic method / Bronwen M. Harvey and Pirkko Soundy -- Capillary electrophoresis of DNA : biomedical applications / Beatriz Sanchez-Vega -- Denaturing high-performance liquid chromatography (DHPLC) for nucleic acid analysis / Kim Hung Leung and Shea Ping Yip -- Denaturing gradient gel electrophoresis (DGGE) / Jeroen H. Roelfsema and Dorien J.M. Peters -- Single strand conformation polymorphism (SSCP) analysis / Kim Hung Leung and Shea Ping Yip -- Randomly amplified polymorphic DNA (RAPD) : a useful tool for genomic characterization of different organisms / Lúcia Maria da Cunha Galvño and Eliane Lages-Silva -- Quantification of mRNA using real time RT-PCR : the SYBR solutiion / David Sugden and Patricia de Winter -- Quantitative analysis of DNA sequences by PCR and solid-phase minisequencing / Anu Suomalainen and Ann-Christine Syvèanen -- Multiplex amplifiable probe hybridization (MAPH) / Carolina Sismani, Ludmila Kousoulidou, and Philippos C. Patsalis -- Gene expression profiling / Arnis Druka, Robbie Waugh, and Pete Hedley -- Comparative genomic hybridization in clinical and medical research / Peng-Hui Wang, Yann-Jang Chen, and Chi-Hung Lin -- Subtractive hybridization / Craig Winstanley -- Fluorescence in situ hybridization / Jane Bayani and Jeremy A. Squire -- Quantitative trait locus mapping to identify genes for complex traits in mice / Jonathan D. Smith -- cDNA microarrays / Phillip G. Febbo -- Mapping techniques / Simon G. Gregory -- Single nucleotide polymorphisms / Nameeta Shah -- Gene/protein sequence analysis : a compilation of bioinformatic tools / Bernd H.A. Rehm and Frank Reinecke -- Protein electrophoresis / David Sheehan and Siobhan O'Sullivan -- Protein blotting / Patricia Gravel -- Capillary electrophoresis of proteins / Mark Strege -- Autoradiograph and fluorography / Bronwen M. Harvey -- Mass spectrometry of proteins and peptides / Kenneth G. Standing -- Post-translational modifications of proteins / Christoph Kannich and Birte Fuchs -- Protein microarray technology / Charlotte H. Clarke and Eric T. Fung -- Protein-protein interactions / Hae Ryoun Park ... [et al.] -- Glycoprotein analysis / Terry D. Butters and David C.A. Neville -- Solid-phase peptide synthesis / Mare Cudic and Gregg B. Fields -- Monoclonal antibodies / Zhong J. Zhang and Maher Albitar -- Antibody phage display / Rob Aitken -- Protein engineering / Thomas Willemsen ... [et al.] -- Directed protein evolution / Sabine C. Stebel ... [et al.] -- Enzyme linked immunosorbent assay (ELISA) / John Crowther -- Epitope mapping : identification of antibody-binding sites on protein antigens / Glenn E. Morris -- Quantum dots / Charles Z. Hotz -- Ion-exchange chromatography / David Sheehan -- Size-exclusion chromatography / Paul Cutler -- Hydrophobic interaction chromatography / Paul A. O'Farrell -- Affinity chromatography / Adam Charlton and Michael Zachariou -- High performance liquid chromatography (HPLC) of peptides and proteins / Tzong-Hsien Lee and Marie-Isabel Aguilar -- Amino acid analysis / Ian Davidson and Paula O'Connor -- Surface plasmon resonance / K. Scott Phillips and Quan Jason Cheng -- Macromolecular crystallography / Bernhard Rupp and Katherine A. Kantardjieff -- Microchip devices for bioanalysis / Anna C. Kinsella and Shelley D. Minteer -- Mammalian cell culture / Simon P. Langdon -- Plant tissue culture / Victor M. Loyola-Vargas ... [et al.] -- Stem cells and regenerative medicine / Mohan C. Vemuri and Chellu S. Chetty -- Cryopreservatioin : conservation of bioresources at ultra low temperatures / John G. Day ... [et al.] -- Magnetic resonance imaging / Pottumarthi v.Prasad and Pippa Storey -- Electron microscopy / John Kuo -- Confocal microscopy / Guy Cox -- Laser microdissection / Graeme I. Murray -- Biomedical uses of flow cytometry / James L. Weaver and Maryalice Stetler-Stevenson -- Immunomicroscopy / Constance Oliver -- In situ hybridization / Ian A. Darby and Tim D. Hewitson -- High throughput screening / William P. Janzen.