TY  - BOOK
AU  - Wang,X.
AU  - Wang,Z.
AU  - Wang,Q.
AU  - Liang,H.
AU  - Liu,D.
TI  - Trichostatin A and vorinostat promote adipogenic differentiation through H3K9 acetylation and dimethylation
KW  - ACETYLATION
KW  - ADIPOGENIC DIFFERENTIATION
KW  - ADIPOSE-DERIVED STEM CELLS
KW  - DIMETHYLATION
KW  - GOAT
N2  - To explore the effect of epigenetic modification on the differentiation of goat adipose-derived stem cells in vitro, we used two common epigenetic modification inhibitors, trichostatin A and vorinostat, to treat cashmere goat adipose-derived stem cells and induce adipocyte differentiation. The results showed that trichostatin A and vorinostat changed the relative amounts of H3K9 acetylation and dimethylation in the upstream sequence of PPARG, increased peroxisome proliferator-activated receptor gamma (PPARG)transcription before differentiation and then promoted adipocyte differentiation, and regulated the expression of adipocyte-specific genes. We conclude that adipocyte differentiation is regulated dynamically by different histone modifications. The areas of acetylation and demethylation changed by trichostatin A and vorinostat are the basis for further research on the mechanism of PPARG promoter to regulate adipocytes differentiation and provide research theroies for using adipose-derived stem cells as donor to produce transgenic animals to improve meat quality improvement
UR  - https://drive.google.com/file/d/1S-lPYqOR5vk2dkl2Ba4VZ1RR3w1u5pH2/view?usp=drivesdk
ER  -