Telomere-associated RFLPs and electrophoretic karyotyping reveal lineage relationship among race-specific strains of Ustilago hordei 

 -  Current Genetics, 38(3), p.414-147, 2000 .
<br/><br/> The inheritance of telomere-associated restriction fragment length polymorphisms (tel-RFLP)and chromosome-length polymorphisms (CLPs)were criteria used for the identification of strains of Ustilago hordei that form a direct lineage. Teliospore collections of race 8 strains and strains reported to be derived from race 8 through inbreeding were used in these analyses. None of the race 8 strains examined in this study, representing three consecutive inbred generations, was polymorphic for any terminal BamHI and BglII chromosomal loci, nor did they have any apparent CLPs. Strains from a teliospore collection obtained in 1971 and designated 447, representing the first inbred generation of race 8 strains and a shift to increased virulence on cultivar Hannchen, had tel-RFLP arrays indistinguishable from the race 8 strains isolated in this study; and they had no obvious CLPs. Strains from the presumed second inbred generation, the 1279 teliospore line, which was pathogenic on six additional cultivars, had numerous CLPs and a tel-RFLP array that differed from race 8 strains at more than 50