TY  - BOOK
AU  - Jacobs,D
AU  - van Rijssen,M.S
AU  - van der Heijsen,R
AU  - Verpoorte,R.
TI  - Sequential solubilization of proteins precipitated with trichloroacetic acid in acetone from cultured Catharanthus roseus cells yields 52 more spots after two-dimensional electrophoresis
N2  - Sample preparation is still the most critical step in two-dimensional gel electrophoresis (2-DE), and needs to be optimized for each type of sample. To analyze the proteome of the medicinal plant Catharanthus roseus, we developed and evaluated a sequential solubilization procedure for the solubilization of proteins after precipitation in trichloroacetic acid and acetone. The procedure includes solubilization with a conventional urea buffer followed by a stronger solubilizing buffer containing thiourea. The sequential solubilization of the precipitated proteins results in very different spot patterns following 2-DE. The number of protein spots which could be detected in both samples of the sequential solubilization was only about 10
UR  - https://drive.google.com/file/d/1IDhK4yTinMNx3FiDKc29t9lFkwSL20Ei/view?usp=drivesdk
ER  -