TY - BOOK AU - Fukami,H. AU - Asakura,T. AU - Hirano,H. AU - Abe,K. AU - Shimomura,K. AU - Yamakawa,T. TI - Salycilic Acid Carboxyl Methyltransferase Induced in Hairy Root Cultures of Atropa belladonna after Treatment with Exogeneously Added Salicylic Acid KW - ATROPA BELLADONNA KW - SALICYLIC ACID KW - METHYL SALICYLATE KW - S-ADENOSYL-L-METHIONINE:SALICYLIC ACID CARBOXYL METHYLTRANSFERASE KW - METHYLTRANSFERASE N2 - In Atropa belladonna hairy roots, exogeneously added salicylic acid (SA)is converted to methyl salicylate (MSA)through the reaction, which might be catalysed by S-adenosyl-L-methionine: salicylic acid carboxyl methyltransferase (SAMT). Here we cloned a cDNA for A. belladonna SAMT (AbSAMT1), which consisted of 357 aa residues. It was expressed in E. coli, and the recombinant AbSAMT1 showed SAMT activity. When A. belladonna hairy roots were exposed to a high concentration of SA, AbSAMT1 mRNA begins to be expressed 12 h after the exposure, and steady expression continued over 144 h UR - https://drive.google.com/file/d/1mcppR6-HBv6qghZDzJtUllfnVvpx76kF/view?usp=drivesdk ER -