TY  - BOOK
AU  - Hatanaka,T.
AU  - Choi,Y.E.
AU  - Kusano,T.
AU  - Sano,H.
TI  - Transgenic plants of coffea canephora from embryogenic callus via agrobacterium tumefaciens-mediated transformation
KW  - COFFEA CANEPHORA
KW  - AGROBACTERIUM TUMEFACIENS
KW  - TRANSFORMATION
N2  - Abstract Embryogenic calli were induced from leaf explants of coffee (Coffea canephora)on McCown's woody plant medium (WPM)supplemented with 5 7M N6-(2-isopentenyl)-adenosine (2-iP). These calli were co-cultured with Agrobacterium tumefaciens EHA101 harboring pIG121-Hm, containing #-glucuronidase (GUS), hygromycin phosphotransferase (HPT), and neomycin phosphotransferase II genes. Selection of putative transgenic callus was performed by gradual increase in hygromycin concentration (5, 50, 100 mg/l). The embryogenic calli surviving on medium containing 100 mg/l hygromycin showed a strong GUS-positive reaction with X-Gluc solution. Somatic embryos were formed from these putative transgenic calli and germinated on WPM medium with 5 7M 2-iP. Regenerated small plantlets with shoots and roots were transferred to medium containing both 100 mg/l hygromycin and 100 mg/l kanamycin for final selection of transgenic plants. The selected plantlets exhibited strong GUS activity in leaves and roots as indicated by a deep blue color. GUS and HPT genes were confirmed to be stably integrated into the genome of the coffee plants by the polymerase chain reaction
UR  - https://drive.google.com/file/d/1oJ4ThleYakSaic7u2MbLHjcc-Wly_VSf/view?usp=drivesdk
ER  -