TY  - BOOK
AU  - Sharp,J.M.
AU  - Doran,P.M.
TI  - Characteristics of growth and tropane alkaloid synthesis in Atropa belladonna roots transformed by Agrobacterium rhizogenes
KW  - AGROBACTERIUM RHIZOGENES
KW  - HAIRY ROOT CULTURE
KW  - BIOCHEMICAL ENGINEERING
KW  - ATROPA BELLADONNA
KW  - ATROPINE
KW  - TROPANE ALKALOIDS
N2  - Growth of Atropa belladonna hairy roots in shake flasks followed first-order kinetics. The specific growth rate was 0.28 d-1, corresponding to a doubling time of 60 h. In airlift reactors, first order kinetics were not observed due to nutrient limitation. Synthesis of tropane alkaloids was growth-associated; no atropine was released into the medium. Sugar concentrations were analyzed during hairy-root culture for calculation of atropine yield. Atropine yields from substrate were 1.4 mg g-1 in shake flasks and 0.46 mg g-1 in airlift reactors. Although levels of atropine in reactor-grown roots (0.37
UR  - https://drive.google.com/file/d/1oq7XS7IPpo2n7VefLSUOopMJTgfPHqhE/view?usp=drivesdk
ER  -