TY  - BOOK
AU  - Potta,G.B.
AU  - Millera,T.K.
AU  - Bartletta,J.A.
AU  - Palasb,J.S.
AU  - Selitrennikoff,C.P.
TI  - The Isolation of FOS-1, a Gene Encoding a Putative Two-Component Histidine Kinase from Aspergillus fumigatus
KW  - ASPERGILLUS FUMIGATUS
KW  - TWO-COMPONENT HISTIDINE KINASE
KW  - DICARBOXIMIDE ANTIFUNGAL COMPOUNDS
KW  - FOS-1
KW  - CELL-WALL ASSEMBLY
N2  - In fungi, two-component histidine kinases are involved in response mechanisms to extracellular changes in osmolarity, resistance to dicarboximide fungicides, and cell-wall assembly. In the human opportunistic fungus, Candida albicans, each of the three histidine kinases plays a role in virulence. Here, we identify, for the first time, a gene, FOS-1, from the human pathogenic fungus Aspergillus fumigatus that predicts a protein with homology to two-component histidine kinases. The predicted FOS-1 protein is highly homologous to bacterial and other fungal histidine kinases in several functional domains, but is divergent at the amino- and carboxy-termini. A mutant lacking the FOS-1 locus, ?FOS-1, did not exhibit a detectable defect in either hyphal growth or morphology when grown on solid or liquid medium. However, in liquid medium, conidiophore development of the ?FOS-1 mutant was delayed. Compared to wild type, the ?FOS-1 strain was neither osmotically sensitive nor sensitive or resistant to a number of nondicarboximide antifungal drugs, but was highly resistant to dicarboximide fungicides and resistant to novozym 234, suggesting that FOS-1p may play a role in the regulation of cell-wall assembly
UR  - https://drive.google.com/file/d/1U5UkZCRAIZhdi1N5kw7TdjfRP6l_WI_s/view?usp=drivesdk
ER  -