Tagging and localization of a phospholipase D gene in Coprinellus congregatus by restriction enzyme-mediated integration and pulsed-field gel electrophoresis 

 -  We have identified a phospholipase D gene (pld)fragment from a transformant generated by restriction enzyme-mediated integration in Coprinellus congregatus, which is a mushroom-forming basidiomycete. A fragment of pld of this fungus has been cloned from a transformant by gene tagging. The transformation vector has been inserted into pld and this has resulted in a decreased enzyme activity of the transformant compared with the wild strain. C. congregatus has seven chromosomes, the range of its genome size is 1.6-4.7 Mb and pld is located at chromosome 4 where the transformation vector has been inserted., 225(2), p.285-289, 2003 .
<br/><br/> We have identified a phospholipase D gene (pld)fragment from a transformant generated by restriction enzyme-mediated integration in Coprinellus congregatus, which is a mushroom-forming basidiomycete. A fragment of pld of this fungus has been cloned from a transformant by gene tagging. The transformation vector has been inserted into pld and this has resulted in a decreased enzyme activity of the transformant compared with the wild strain. C. congregatus has seven chromosomes, the range of its genome size is 1.6-4.7 Mb and pld is located at chromosome 4 where the transformation vector has been inserted. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>PHOSPHOLIPASE D<br/>GENE TAGGING<br/>PLASMID RESCUE<br/>PULSED-FIELD GEL ELECTROPHORESIS