TY  - BOOK
AU  - Leema,Y.E.
AU  - Rossb,I.K.
AU  - Choi,H.T.
TI  - Tagging and localization of a phospholipase D gene in Coprinellus congregatus by restriction enzyme-mediated integration and pulsed-field gel electrophoresis
KW  - PHOSPHOLIPASE D
KW  - GENE TAGGING
KW  - PLASMID RESCUE
KW  - PULSED-FIELD GEL ELECTROPHORESIS
N2  - We have identified a phospholipase D gene (pld)fragment from a transformant generated by restriction enzyme-mediated integration in Coprinellus congregatus, which is a mushroom-forming basidiomycete. A fragment of pld of this fungus has been cloned from a transformant by gene tagging. The transformation vector has been inserted into pld and this has resulted in a decreased enzyme activity of the transformant compared with the wild strain. C. congregatus has seven chromosomes, the range of its genome size is 1.6-4.7 Mb and pld is located at chromosome 4 where the transformation vector has been inserted
UR  - https://drive.google.com/file/d/1Rye-L45WD2vXkW0WYrJEsAgOBgomidb6/view?usp=drivesdk
ER  -