Responses of transformed root culture of Atropa belladonna to salicylic acid stress
- Journal of BioScience and BioEngineering, 91(6), p.586-589, 2001 .
The effect of salicylic acid (SA)on tropane alkaloid production and the responses to SA stress of transformed root cultures of Atropa belladonna (belladonna)were investigated. Treatment of A. belladonna transformed roots with 0.2 mM SA did not have any effect on tropane alkaloid production, but two compounds were produced in the medium. These were identified as the SA derivatives methylsalicylate and methyl-o-methoxybenzoate by high-resolution mass spectrometry and UV spectrometry. In contrast, treatment with 2 mM salicylic acid stimulated tropane alkaloid release from the transformed roots into the medium by up to 35