TY  - BOOK
AU  - Arnold,D.L.
AU  - Jackson,R.W.
AU  - Fillingham,A.J.
AU  - Goss,S.C.
AU  - Taylor,J.D.
AU  - Mansfield,J.W.
AU  - Vivian,A.
TI  - Highly conserved sequences flank avirulence genes: isolation of novel avirulence genes from Pseudomonas syringae pv. pisi
KW  - PSEUDOMONAS SYRINGAE
KW  - CONSERVED DNA SEQUENCES
KW  - AVR
KW  - PLASMID
KW  - RULAB
N2  - DNA sequences flanking two avr genes (avrPpiA1 and avrPpiB1)from Pseudomonas syringae pv. pisi show a high degree of similarity. Specific primers designed from the conserved regions were used in PCR amplifications with all P. syringae pv. pisi races. As well as amplifying the expected avrPpiA- and avrPpiB-containing fragments, two additional fragments were amplified: one contained a single open reading frame (ORF1)and was found in races of genomic group II (2, 3A, 4A and 6); the second fragment contained two open reading frames (ORF2 and ORF3), separated by 658 nt, and was detected in all races. All three ORFs had G+C ratios (46·9-48 mol
UR  - https://drive.google.com/file/d/19OnoeppNb_blz9CsinjifrTYkdyXH0bL/view?usp=drivesdk
ER  -