TY  - BOOK
AU  - Kamada,K.
AU  - Shu,F.
AU  - Chen,H.
AU  - Malik,S.
AU  - Stelzer,G.
AU  - Roeder,R.G.
AU  - Meisterernst,M.
AU  - Meisterernst,M.
TI  - Crystal Structure of Negative Cofactor 2 Recognizing the TBP-DNA Transcription Complex
N2  - The X-ray structure of a ternary complex of Negative Cofactor 2 (NC2), the TATA box binding protein (TBP), and DNA has been determined at 2.6 A° resolution. The N termini of NC2 a and ß resemble histones H2A and H2B, respectively, and form a heterodimer that binds to the bent DNA double helix on the underside of the preformed TBP-DNA complex via electrostatic interactions. NC2ß contributes to inhibition of TATA-dependent transcription through interactions of its C-terminal a helix with a conserved hydrophobic feature on the upper surface of TBP, which in turn positions the penultimate a helix of NC2ß to block recognition of the TBP-DNA complex by transcription factor IIB. Further regulatory implications of the NC2 heterodimer structure are discussed
UR  - https://drive.google.com/file/d/18TkaTCYHbGexUAQhKdYuwmUwR8GwdbvA/view?usp=drivesdk
ER  -