TY  - BOOK
AU  - Yun,S.-H.
AU  - Turgeon,B.G.
AU  - Yoder,O.C.
TI  - REMI-induced mutants ofMycosphaerella zeae-maydislacking the polyketide PM-toxin are deficient in pathogenesis to corn
N2  - The polyketide PM-toxin, produced byMycosphaerella zeae-maydis, is specifically active against corn containing Texas male sterile (T)cytoplasm, as is the fungus itself. To determine if PM-toxin is required for disease development,Tox-mutants were generated using the restriction enzyme-mediated integration (REMI)strategy, which is designed to tag the mutations it creates. As a mutagenic procedure, REMI was highly efficient, yielding five stable PM-toxin-deficient mutants among 504 transformants recovered. AllTox-mutants lost the ability to cause disease on T-cytoplasm corn, establishing a role for PM-toxin in pathogenesis. Examination of genomic DNAs from theTox-mutants revealed one that carried the transformation vector at multiple insertion sites and two that had no apparent ORFs at single vector insertion sites; three sustained deletions
UR  - https://drive.google.com/file/d/1RuHyuwPt8LtxPDX5v5xeHKm7k5eQeTyw/view?usp=drivesdk
ER  -