An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and Red 

 -  FEMS MicroBiology Letters , 234(2), p.325-331 , 2004 .
<br/><br/> We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems, Red and Cre/loxP. The loxP site could be rapidly and efficiently integrated in the genome by Red and large deletions of both 117- and 165-kbp regions could be generated in 100 
<br/><br/><label>Subjects--Topical Terms: </label><br/>ESCHERICHIA COLI<br/>GENOME ENGINEERING<br/>CRE/LOXP<br/>RED<br/>LARGE DELETION