TY  - BOOK
AU  - Fukiya,S.
AU  - Mizoguchi,H.
AU  - Mori,H.
TI  - An improved method for deleting large regions of Escherichia coli K-12 chromosome using a combination of Cre/loxP and Red
KW  - ESCHERICHIA COLI
KW  - GENOME ENGINEERING
KW  - CRE/LOXP
KW  - RED
KW  - LARGE DELETION
N2  - We have established an improved large deletion method in Escherichia coli genome using a combination of two different recombination systems, Red and Cre/loxP. The loxP site could be rapidly and efficiently integrated in the genome by Red and large deletions of both 117- and 165-kbp regions could be generated in 100
UR  - https://drive.google.com/file/d/1hMWfsXY8WbvEFhrQwsutuWr7UcODDUdj/view?usp=drivesdk
ER  -