A Method for Isolation of Total RNA from Fruit Tissues of Banana 

 -  Plant Molecular Biology Reporter, 16, p.1-6, 1998 .
<br/><br/> We describe a rapid and efficient method for isolation of total RNA from banana fruit tissues. The RNA was extracted with a high ionic strength buffer at room temperature. The proteins, genomic DNA and secondary metabolites in the extract were then removed by precipitation with pre-cooled potassium acetate and repeated phenol/chloroform/isoamyl alcohol extractions. The RNA was recovered by ethanol precipitation. It was relatively free of ribonucleases and was suitable for RT-PCR and northern blot analysis. The procedure can be completed in less than 4 hours. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>BANANA<br/>FRUIT TISSUE<br/>MUSA SPP.<br/>RNA ISOLATION