TY  - BOOK
AU  - Liu,J.J.
AU  - Goh,C-J.
AU  - Loh,C-S.
AU  - Liu,P.
TI  - A Method for Isolation of Total RNA from Fruit Tissues of Banana
KW  - BANANA
KW  - FRUIT TISSUE
KW  - MUSA SPP
KW  - RNA ISOLATION
N2  - We describe a rapid and efficient method for isolation of total RNA from banana fruit tissues. The RNA was extracted with a high ionic strength buffer at room temperature. The proteins, genomic DNA and secondary metabolites in the extract were then removed by precipitation with pre-cooled potassium acetate and repeated phenol/chloroform/isoamyl alcohol extractions. The RNA was recovered by ethanol precipitation. It was relatively free of ribonucleases and was suitable for RT-PCR and northern blot analysis. The procedure can be completed in less than 4 hours
UR  - https://drive.google.com/file/d/1HVf8nO4Kiuf_Ch4Aj_gDGAOWFi6jklgL/view?usp=drivesdk
ER  -