Protoplast preparation and transient transformation of Rhizoctonia solani 

 -  Mycological Research, 105, p.1295-1303, 2001 .
<br/><br/> Optimization of protoplast release (202×104 ml[minus sign]1)from a strain of Rhizoctonia solani anastomosis group three was achieved by treating 3 d old mycelia from broth cultures with Novozyme 234 for 3 h at 30 °C. The only other treatment, of many tested, that yielded any protoplasts (15·6×104 ml[minus sign]1)was commercial lysing enzymes from Trichoderma harzianum. Various combinations of commercial enzyme preparations, variations of osmotic stabilisers, and mycelial age, were ineffective in raising the protoplast yield. Regeneration of colonies from protoplasts was maximal (83-87