TY  - BOOK
AU  - Schwender,J.
AU  - Muller,Ch
AU  - Zeidler,J.
AU  - Lichtenthaler,H.K.
TI  - Cloning and heterologous expression of a cDNA encoding l-deoxy-D-xylulose-5-phosphate reductoisomerase of Arabidopsis thaliana1
KW  - 1-DEOXY-D-XYLULOSE
KW  - 1-DEOXY-D-XYLULOSE 5-PHOSPHATE REDUCTOISOMERASE
KW  - 1-DEOXY-D-XYLULOSE 5-PHOSPHATE PATHWAY
KW  - ISOPRENOID BIOSYNTHESIS
KW  - 2-C-METHYL-D-ERYTHRITOL
N2  - Various plant isoprenoids are synthesized via the nonmevalonate pathway of isopentenyl diphosphate formation. In this pathway, 1-deoxy-D-xylulose 5-phosphate (DOXP), the first intermediate, is transformed to 2-C-methyl-D-erythritol 4-phosphate (MEP)by an enzyme which was recently cloned from Escherichia coli. In order to find a plant homologue of this 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR)we cloned a cDNA fragment from Arabidopsis thaliana which has high homology to the E. coli DXR. By expression of this fragment in E. coli we could demonstrate that it encodes a protein which transforms DOXP to MEP. The antibiotic fosmidomycin pecifically inhibits this DXR enzyme activity
UR  - https://drive.google.com/file/d/17fLBAMxYhkDtTXhu0XupuduP3K_OKVMl/view?usp=drivesdk
ER  -