TY  - BOOK
AU  - Mizunoa,K.
AU  - Okuda,A.
AU  - Kato,M.
AU  - Yoneyama,N.
AU  - Tanaka,H.
AU  - Ashihara,H.
AU  - Fujimura,T
TI  - Isolation of a new dual-functional ca¡eine synthase gene encoding an enzyme for the conversion of 7-methylxanthine to ca¡eine from coffee (Coffea arabica L.)1
KW  - CO¡EE
KW  - CA¡EINE
KW  - N-METHYLTRANSFERASE
KW  - 7-METHYLXANTHINE
KW  - S-ADENOSYL-L-METHIONINE
KW  - THEOBROMINE
N2  - In co¡ee and tea plants, ca¡eine is synthesized from xanthosine via a pathway that includes three methylation steps. We report the isolation of a bifunctional co¡ee ca¡eine synthase (CCS1)clone from co¡ee endosperm by reverse transcriptionpolymeras chain reaction (RT-PCR)and rapid ampli¢cation of cDNA ends (RACE)technique using previously reported sequence information for theobromine synthases (CTSs). The predicted amino acid sequences of CCS1are more than 80
UR  - https://drive.google.com/file/d/1y6v-JV0a1C_uMO09hd04PBhHc4G-DIw_/view?usp=drivesdk
ER  -