TY  - BOOK
AU  - Loiselle,D.R.
AU  - Thelin,W.R.
AU  - Parker,C.E.
AU  - Dicheva,N.D.
AU  - Kesner,B.A.
AU  - Mocanu,V.
AU  - Wang,F.
AU  - Wang,F.
AU  - Esteban Warren,M.R.
AU  - Borchers,C.H.
TI  - Improved Protein Identification through the Use of Unstained Gels
KW  - UNSTAINED GELS
KW  - PROTEIN IDENTIFICATION
KW  - PAGE
KW  - SILVER STAINING
KW  - IMMUNOPRECIPITATION
KW  - ROBOTICS
KW  - AUTOMATION
KW  - MALDIMS
N2  - In this work, a method for improved protein identification of low-abundance proteins using unstained gels, in combination with robotics and matrix-assisted laser desorption/ionization tandem mass spectrometry, has been developed and evaluated. Omitting the silverstaining process resulted in increased protein identification scores, an increase in the number of peptides observed in the MALDI mass spectrum, and improved quality of the tandem mass spectrometry data
UR  - https://drive.google.com/file/d/1Jp7fDqCjsST2gxGLGNyE4jp8i81XsUmj/view?usp=drivesdk
ER  -