Proteomic analysis of secreted proteins from Trichoderma harzianum Identification of a fungal cell wall-induced aspartic protease 

 -  Fungal Genetics and Biology, 42(11), p.924-934, 2005 .
<br/><br/> Trichoderma mycoparasitic activity depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall. We have analysed the extracellular proteome secreted by T. harzianum CECT 2413 in the presence of different fungal cell walls. Significant differences were detected in 2DE maps, depending on the use of specific cell walls or chitin. A combination of MALDITOF and liquid chromatography mass spectrometry allowed the identification of a novel aspartic protease (P6281: MW 33 and pI 4.3)highly induced by fungal cell walls. A broad EST library from T. harzianum CECT 2413 was used to obtain the full-length sequence. The protein showed 44 
<br/><br/><label>Subjects--Topical Terms: </label><br/>ASPARTIC PROTEASE<br/>BIOCONTROL<br/>EXPRESSED SEQUENCE TAGS<br/>FILAMENTOUS FUNGI PROTEOMICS<br/>GENE EXPRESSION<br/>LC/MS-MS<br/>MALDI-TOF<br/>MYCOPARASITISM<br/>TRICHODERMA HARZIANUM