TY  - BOOK
AU  - Suárez,M.B.
AU  - Sanz,L.
AU  - Chamorro,M.I.
AU  - Rey,M.
AU  - González,F.J.
AU  - Llobell,A.
AU  - Monte,E.
TI  - Proteomic analysis of secreted proteins from Trichoderma harzianum Identification of a fungal cell wall-induced aspartic protease
KW  - ASPARTIC PROTEASE
KW  - BIOCONTROL
KW  - EXPRESSED SEQUENCE TAGS
KW  - FILAMENTOUS FUNGI PROTEOMICS
KW  - GENE EXPRESSION
KW  - LC/MS-MS
KW  - MALDI-TOF
KW  - MYCOPARASITISM
KW  - TRICHODERMA HARZIANUM
N2  - Trichoderma mycoparasitic activity depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall. We have analysed the extracellular proteome secreted by T. harzianum CECT 2413 in the presence of different fungal cell walls. Significant differences were detected in 2DE maps, depending on the use of specific cell walls or chitin. A combination of MALDITOF and liquid chromatography mass spectrometry allowed the identification of a novel aspartic protease (P6281: MW 33 and pI 4.3)highly induced by fungal cell walls. A broad EST library from T. harzianum CECT 2413 was used to obtain the full-length sequence. The protein showed 44
UR  - https://drive.google.com/file/d/1f6rX0mCaYkI2pNw7PQeK61SYJcIOSFR1/view?usp=drivesdk
ER  -