TY  - BOOK
AU  - Sullivan,M.L.
AU  - Hatfield,R.D.
AU  - Thoma,S.L.
AU  - Samac,D.A.
TI  - Cloning and Characterization of Red Clover Polyphenol Oxidase cDNAs and Expression of Active Protein in Escherichia coli and Transgenic Alfalfa1[w]
N2  - Red clover (Trifolium pratense)leaves contain high levels of polyphenol oxidase (PPO)activity and o-diphenol substrates. Wounding of leaves during harvest and ensiling results in browning of leaf tissues from activity of PPO on the o-diphenols. In association with browning, leaf proteins remain undegraded during ensiling, presumably due to PPO-generated o-quinone inhibition of leaf proteases. We cloned three red clover PPO cDNAs, PPO1, PPO2, and PPO3, from a leaf cDNA library. Sequence comparisons among the three red clover PPO clones indicated they are 87
UR  - https://drive.google.com/file/d/18xfyKpHNCv7uJN8mXdO4CzvUs6dR5nMV/view?usp=drivesdk
ER  -