ISSR-PCR technique: a useful method for characterizing new allotetraploid somatic hybrids of mandarin 

 -  Plant Cell Reports, 20, p.1162-1166, 2002 .
<br/><br/> Somatic hybrids between Redblush and Duncan grapefruit (Citrus paradisi Macfadyen)with Avana, Tardivo di Ciaculli mandarin (C. deliciosa Tenore)and Fortune mandarin (C. reticulata Blanco)were obtained by symmetric protoplast fusion. Isozyme banding pattern analysis and flow cytometry were used for early screening. DNA of the hybrids was then extracted and subjected to inter-simple sequence repeat polymerase chain reaction (ISSR-PCR)using different primers anchored at the 5_O and 3_O ends. PCR products were resolved on 1.5