TY - BOOK AU - Joarder,O.I. AU - Joarder,N.H. AU - Dale,P.J. TI - In vitro response of leaf tissues from Lolium multifloruma comparison with leaf segment position, leaf age and in vivo mitotic activity KW - CALLUS KW - LEAF CULTURE KW - MITOTIC INDEX KW - LOLIUM MULTIFLORUM KW - ITALIAN RYEGRASS N2 - Immature gramineous leaves provide a convenient system for comparing the response of cells in culture with their state of differentiation. Callusing frequency is compared with leaf segment position, leaf age and in vivo mitotic activity in Lolium multiflorum. (1)In a succession of one millimeter sections from the immature leaf base, callus was formed from the first and second sections but not the third or subsequent sections. The frequency of those explants callusing decreased with distance from the base of the leaf and with leaf age (or leaf extension growth). (2)In vivo, the proportion of cells in mitosis declined from around 10-14 UR - https://drive.google.com/file/d/11B_fztcGPuUkhn3Y6b9XMTi1d6WEvW2Y/view?usp=drivesdk ER -