TY  - BOOK
AU  - Mizoguchi,T.
AU  - Ichimura,K.
AU  - Irie,K.
AU  - Morris,P.
AU  - Giraudat,J.
AU  - Matsumoto,K.
AU  - Shinozaki,K.
TI  - Identi¢cation of a possible MAP kinase cascade in Arabidopsis thaliana based on pairwise yeast two-hybrid analysis and functional complementation tests of yeast mutants
KW  - MITOGEN-ACTIVATED PROTEIN KINASE CASCADE
KW  - YEAST TWO-HYBRID
KW  - TRANS-COMPLEMENTATION
KW  - ARABIDOPSIS THALIANA
N2  - A possible MAP kinase (MAPK)cascade of Arabidopsis thaliana was identified on the basis of both yeast 2-hybrid analysis and complementation analysis of yeast mutants. Specific protein-protein interactions between ATMPK4 (a MAPK)and MEK1 (a MAPKK)and interactions between MEK1 and ATMEKK1 (a MAPKKK)were detected by using the 2-hybrid system. A growth defect of the yeast mpk1v mutant was reversed by coexpression of ATMPK4 and MEK1. Coexpression of the N-terminal deletion form of ATMEKK1 increased the ability of MEK1 to suppress a growth defect of the yeast pbs2v mutant. These results suggest that ATMPK4, MEK1, and ATMEKK1 may interact with each other and constitute a specific MAPK cascade in Arabidopsis. This is the first demonstration of a possible MAPK cascade in plants
UR  - https://drive.google.com/file/d/1NqgOm4YG9w44icEbG7g-rAC-p25j4iCl/view?usp=drivesdk
ER  -