TY  - BOOK
AU  - Gao,H.
AU  - Lu,J.
AU  - Cui,Y.
AU  - Zhang,X.X.
TI  - Electrochemical immunoassay of estrone at an antibody-modified conducting polymer electrode towards immunobiosensors
KW  - ESTRONE
KW  - POLYPYRROLE
KW  - FERROCYANIDE
KW  - IMMUNOASSAY
KW  - ELECTROCHEMICAL DETECTION
N2  - A simple electrochemical immunoassay based on an antibody-modified conducting polymer electrode by direct electrochemical polymerization of pyrrole on a platinum electrode in an aqueous solution and simultaneous doping of the polymer with anti-estrone antibody as the dopant was demonstrated. The specific binding between estrone and anti-estrone antibody immobilized on the electrode surface was evaluated by monitoring the changes in the electrode response of potassium ferrocyanide as the redox marker and a decreased electrode response was observed upon the binding of the estrone to the polypyrrole-antibody (PPy-Ab)modified electrode surface. This change was attributed to the steric hindrance generated from the formation of immunological complex between the electrode surface and the redox marker. A direct and facile method of electrochemical detection of estrone has been established in this way
UR  - https://drive.google.com/file/d/1t-DyQFEOtVIYk8FW9VoBlECtBqzGaNE4/view?usp=drivesdk
ER  -