TY  - BOOK
AU  - Katoh,A.
AU  - Uenohara,K.
AU  - Akita,M.
AU  - Hashimoto,T.
TI  - Early Steps in the Biosynthesis of NAD in Arabidopsis Start with Aspartate and Occur in the Plastid1
N2  - NADis a ubiquitous coenzyme involved in oxidation-reduction reactions and is synthesized by way of quinolinate. Animals and some bacteria synthesize quinolinate from tryptophan, whereas other bacteria synthesize quinolinate from aspartate (Asp)using L-Asp oxidase and quinolinate synthase. We show here that Arabidopsis (Arabidopsis thaliana)uses the Asp-to-quinolinate pathway. The Arabidopsis L-Asp oxidase or quinolinate synthase gene complemented the Escherichia coli mutant defective in the corresponding gene, and T-DNA-based disruption of either of these genes, as well as of the gene coding for the enzyme quinolinate phosphoribosyltransferase, was embryo lethal. An analysis of functional green fluorescent protein-fused constructs and in vitro assays of uptake into isolated chloroplasts demonstrated that these three enzymes are located in the plastid
UR  - https://drive.google.com/file/d/1ZRFcb4kviNXuBeIM79Juo4VhZbKUyc96/view?usp=drivesdk
ER  -