Identification of Isoquinoline Alkaloids in Crude Extracts by High Temperature Gas Chromatography-Mass Spectrometry 

 -  PhytoChemical Analysis, 10(5), p.254-258, 1999 .
<br/><br/> Crude ethanol extracts of leaves of Croton (Euphorbiaceae)species (C. hemiargyreus var. gymnodiscus, C. hemiargyreus var. hemiargyreus and C. echinocarpus)were analysed using on-column injection high temperature gas chromatography coupled with mass spectrometry. Several isoquinoline alkaloids were identified, including isocorydine, corydine, glaucine and laurelliptine as the principal constituents. The cold on-column technique proved to be appropriate for injection of these compounds. The technique is shown to be a valuable tool and an alternative technique to classical phytochemical procedures permitting the fast analysis of alkaloid mixtures without the need for (extensive)fractionation and derivatisation. 
<br/><br/><label>Subjects--Topical Terms: </label><br/>COLD ON-COLUMN INJECTOR<br/>HIGH TEMPERATURE GAS CHROMATOGRAPHY<br/>ALKALOIDS<br/>EUPHORBIACEAE<br/>CROTON