TY  - BOOK
AU  - Bergero,R.
TI  - AT-rich sequences from the arbuscular mycorrhizal fungus Gigaspora rosea exhibit ARS function in the yeast Saccharomyces cerevisiae
KW  - GLOMALES
KW  - REPETITIVE SEQUENCES
KW  - REPLICATIVE PLASMID
KW  - ORIGIN OF REPLICATION
KW  - TERMINAL INVERTED REPEATS
KW  - MITES
N2  - Autonomous replicating sequences are DNA elements that trigger DNA replication and are widely used in the development of episomal transformation vectors for fungi. In this paper, a genomic library from the mycorrhizal fungus Gigaspora rosea was constructed in the integrative plasmid YIp5 and screened in the budding yeast Saccharomyces cerevisiae for sequences that act as ARS and trigger plasmid replication. Two genetic elements (GrARS2, GrARS6)promoted high-rates of yeast transformation. Sequence analysis of these elements shows them to be AT-rich (72-80
UR  - https://drive.google.com/file/d/1j1AB1HF0qO_f7Jp_cLT6dYAXiDc8gY0M/view?usp=drivesdk
ER  -