TY - BOOK AU - Wu,W. AU - Pang,Y. AU - Shen,G. AU - Lu,J. AU - Lin,J. AU - Wang,J. AU - Sun,X. AU - Sun,X. TI - Molecular Cloning, Characterization and Expression of a Novel Trehalose-6-phosphate Synthase Homologue from Ginkgo biloba KW - CDNA CLONING KW - GBTPS KW - GINKGO BILOBA KW - RACE KW - TREHALOSE N2 - In many organisms, trehalose acts as protective metabolite against harsh environmental stresses, such as freezing, drought, nutrient starvation, heat and salt. Herein a cDNA (designated as GbTPS, GenBank Accession Number AY884150)encoding a trehalose-6-phosphate synthase homologue was isolated and characterized from the living fossil plant, Ginkgo biloba, which is highly tolerant to drought and cold. GbTPS encoded an 868-amino-acid polypeptide with a predicted isoelectric point of 5.83 and molecular mass of 97.9 kD. Amino acid sequence alignment revealed that GbTPS shared high identity with class II trehalose-6-phosphate synthase homologues (67 UR - https://drive.google.com/file/d/1Ay5WLrIrOl6wuWw3bMOXf8SMEG7HcWDn/view?usp=drivesdk ER -