TY  - BOOK
AU  - Wirth,S.J.
AU  - Wolf,G.A.
TI  - Dye-labelled substrates for the assay and detection of chitinase and lysozyme activity
KW  - CHITINASE ASSAY
KW  - CHITIN DERIVATIVE
KW  - PLATE-CLEARING ASSAY
KW  - REMAZOL BRILLIANT VIOLET 5R
N2  - A carboxymethyl-substituted soluble chitin and a reprecipitated colloidalchitin derivative were covalently linked with Remazol Brilliant Violet 5R. Both conjugates are new dye-labelled substrates suitable for the assay and detection of chitinase and lysozyme activity. A reliable colorimetric microassay adapted to microtitre plates was established, based on the precipitability of carboxymethyl-chitin-Remazol Brilliant Violet 5R from buffered solutions with hydrochloric acid. Furthermore, sensitive plate-clearing assays were developed for the isolation and detection of chitinolytic microbial populations, and for the screening of pure cultures
UR  - https://drive.google.com/file/d/1PMNqjdYjnoNc741w-ZXMiJQ1v6hWGB3G/view?usp=drivesdk
ER  -