Downregulation of Solanum americanum genes encoding proteinase inhibitor II causes defective seed development - The Plant Journal, 45(1), p.58-70, 2006 .

Proteinase inhibitor II proteins (PIN2)are serine proteinase inhibitors found in the Solanaceae. Here, we assign functions in seed development to two Solanum americanum genes, SaPIN2a and SaPIN2b, encoding proteinase inhibitor II. Their mRNAs and proteins have been previously ocalized to the reproductive tissues, including the inner cell layers of ovules in senescent flowers at the beginning of fruit development, uggestive of their endogenous roles in reproductive development. We have employed RNA interference (RNAi)-induced post-transcriptional gene silencing (PTGS)to further investigate the role of SaPIN2a and SaPIN2b during seed development. A SaPIN2a-derived construct that shared 83


OVULE
PROTEINASE INHIBITOR
RNA INTERFERENCE
SEED ABORTION
SEED COAT ENDOTHELIUM
SOLANACEAE