Molecular cloning, characterization, and overexpression of a novel [Fe]-hydrogenase isolated from a high rate of hydrogen producing Enterobacter cloacae IIT-BT 08 

 -  BioChemical and Biophysical Research Communcations, 324(2), p.679-685, 2004 .
<br/><br/> Degenerate primers were designed from the conserved zone of hydA structural gene encoding for catalytic subunit of [Fe]-hydrogenase of different hydrogen producing bacteria. A 750 bp of PCR product was amplified by using the above-mentioned degenerate primers and genomic DNA of Enterobacter cloacae IIT-BT 08 as template. The amplified PCR product was cloned and sequenced. The sequence showed the presence of an ORF of 450 bp with significant similarity (40 
<br/><br/><label>Subjects--Topical Terms: </label><br/>ENTEROBACTER CLOACAE<br/>[FE]-HYDROGENASE<br/>HYDA<br/>H-CLUSTER<br/>ANAEROBE