TY  - BOOK
AU  - Mishra,J.
AU  - Khurana,S.
AU  - Kumar,N.
AU  - Ghosh,A.K.
AU  - Das,D.
TI  - Molecular cloning, characterization, and overexpression of a novel [Fe]-hydrogenase isolated from a high rate of hydrogen producing Enterobacter cloacae IIT-BT 08
KW  - ENTEROBACTER CLOACAE
KW  - [FE]-HYDROGENASE
KW  - HYDA
KW  - H-CLUSTER
KW  - ANAEROBE
N2  - Degenerate primers were designed from the conserved zone of hydA structural gene encoding for catalytic subunit of [Fe]-hydrogenase of different hydrogen producing bacteria. A 750 bp of PCR product was amplified by using the above-mentioned degenerate primers and genomic DNA of Enterobacter cloacae IIT-BT 08 as template. The amplified PCR product was cloned and sequenced. The sequence showed the presence of an ORF of 450 bp with significant similarity (40
UR  - https://drive.google.com/file/d/1CX_YmRR-NCeXAGp0-NnQkYsDAAj77hGL/view?usp=drivesdk
ER  -