Intracellular localization of the hydrogenase in Desulfotomaculum orientis 

 -  FEMS MicroBiology Letters, 36(2-3), p.257, 1986 .
<br/><br/> Cell extracts of Desulfotomaculum orientis, grown with H 2 plus sulfate as sole energy source, revealed hydrogenase activities between 0.3 and 2 #mol H 2 per min and nag protein when methyl viologen was used as electron acceptor. With benzyl viologen, methylene blue, FAD or FMN, lower activities were found; NAD was not reduced. The hydrogenase activity was strongly inhibited by CuC12; however, copper inhibition was not observed with whole cells, indicating that the hydrogenase is located intracellularly. After high-speed centrifugation of cell-free extracts, varying proportions, between 11 and 90