TY  - BOOK
AU  - Cypionka,H.
AU  - Dilling,W.
TI  - Intracellular localization of the hydrogenase in Desulfotomaculum orientis
N2  - Cell extracts of Desulfotomaculum orientis, grown with H 2 plus sulfate as sole energy source, revealed hydrogenase activities between 0.3 and 2 #mol H 2 per min and nag protein when methyl viologen was used as electron acceptor. With benzyl viologen, methylene blue, FAD or FMN, lower activities were found; NAD was not reduced. The hydrogenase activity was strongly inhibited by CuC12; however, copper inhibition was not observed with whole cells, indicating that the hydrogenase is located intracellularly. After high-speed centrifugation of cell-free extracts, varying proportions, between 11 and 90
UR  - https://drive.google.com/file/d/13SNUdOLVkM4gt_wuFQsa5YVEBeZCW5a-/view?usp=drivesdk
ER  -