TY  - BOOK
AU  - Gupta,P.K.
AU  - Timmis,R.
TI  - Mass propagation of conifer trees in liquid cultures-progress towards commercialization
KW  - ABA
KW  - ABSCISIC ACID
KW  - ESM-EMBRYONAL SUSPENSOR MASS
KW  - GA-GIBBERELLIC ACID
KW  - PEG-POLYETHYLENE GLYCOL
N2  - At Weyerhaeuser Company, somatic embryo production in liquid medium and manufactured seed delivery has been developed to reduce labor costs and increase efficiency of mass clonal propagation. We have scaled-up embryonal suspensor masses (ESM)of Douglas-fir in 1-l liquid medium flasks. Over 10,000 somatic embryos have been produced from a single flask. We have cryostored ESM of over 700 genotypes in liquid nitrogen. Somatic embryos of Douglas-fir have also been produced from liquid medium in a bioreactor. Different types of bioreactors are required for embryo multiplication and for cotyledonary embryo development. Over 250,000 Douglas-fir somatic seedlings from a large number of enotypes have been produced for clonal field tests
UR  - https://drive.google.com/file/d/1GoXFxyQQRFEW3tRdn4psnC9jgyLSptTe/view?usp=drivesdk
ER  -